Head of the Clinic: Prof. Dr. Nijaz Tihić

Telephone: ++ 387 35 303 512

Head technician: Sadik Jahić, MSc., engineer of medical laboratory diagnostics

Telephone: ++ 387 35 303 547
E-mail: sadik.jahic@ukctuzla.ba

Quality improvement and education: Mirela Salihović, graduated nurse

Telephone: ++ 387 35 303 515
E-mail: mirela.salihovic@ukctuzla.ba

Contact telephones:

Institute for pathology

Phone: ++ 387 35 303 542- Head of institute
Phone: ++ 387 35 303 515 i 303 547-Nurse/ technician in charge
Phone: ++387 35 303 534- Information on findings 2 pm- 3:30 pm
Phone: ++ 387 35 303 515- Receiving samples
Phone: ++ 387 35 303 357- Taking over the deceased (8 am- 4 pm)

Institute for microbiology

Phone: ++ 387 35 303 536- Head of institute
Phone: ++ 387 35 303 568- Nurse/ technician in charge
Phone: ++ 387 35 303 517- Laboratory

Admission department:
Phone: ++ 387 35 303 511
Information on findings:
Phone: ++ 387 35 303 511 (12 noon- 2:30 pm)

Institute for biochemistry

Phone: ++ 387 35 303 502- Head of institute
Phone: ++ 387 35 303 525- Nurse/ technician in charge
Phone: ++ 387 35 303 516 – Laboratory for urgent cases
Phone: ++ 387 35 303 571- Information on findings

History

The Polyclinic for laboratory diagnostic was established in 2003 after consolidating and merging departments of pathology, microbiology and biochemistry into one unit.

Health care services

  • Diagnostic services in the field of pathology, cytology, flow cytometry, cytogenetics, molecular diagnostics, microbiological and biochemical diagnostics.
  • Participation in activities of oncology councils, kidney and liver transplantation councils and council for hematopoietic stem cell transplantation.
  • Diagnostic services quality and safety improvement and quality
  • Teaching and education for students of health faculties, interns and specialist
  • Scientific and research activities
  • Specialist and clinical laboratory consultations and supervision of laboratory diagnostic in the Tuzla Canton.

    ORGANIZATIONAL STRUCTURE

INSTITUTE FOR PATHOLOGY

Chief of institute: Prof. Dr. Ermina Iljazović

Phone: ++ 387 35 303 542
E-mail: ermina.iljazovic@ukctuzla.ba

Nurse in charge: Mersiha Kalesić, engineer of medical laboratory diagnostics

Phone: + 387 35 303 515; 303 574

e-mail: mersiha.kalesic@ukctuzla.ba

HEALTH CARE SERVICES

Processing and standard microscopic diagnosis of small tissue samples obtained by endoscopic techniques, needle biopsy, surgical biopsies, as well as samples obtained after surgical interventions. Processing and microscopic diagnosis of surgical samples of fresh tissue on cryostat for urgent intraoperative analyses.

  • Special histochemical stains and immunohistochemical stains with more than 200 specific antibodies

  • Processing and analysis of aspiration and exfoliative cytological samples

  • DNA typing of HLA antigens class I and II by PCR-SSOP, PCR-SSP and sequence analysis method (PCR-SBT)

  • DNA profile determination by analysis of 15 STR gene loci and the amelogenin (16 STR loci)

  • Identification of 19 mutations on the CFTR gene for molecular diagnosis of cyst fibrosis

  • Identification and typing of human papillomavirus (HPV) in cervical smear

  • Chromogenic in situ hybridization(CISH) and fluorescent in situ hybridization(FISH) for the evaluation of HER2 amplification in breast cancer tissue samples

  • Fluorescence in situ hybridization for diagnosis of chromosomal aberrations

  • Analysis of the constitutive karyotype by the G banding method

  • Qualitative RT PCR to prove the expression of BCR/ABL and PLM/RAR fusion genes

  • Qualitative analysis of genes responsible for resistance to imatinib mesylate (Gleevek)

  • Flow cytometric analysis of the immunophenotype of peripheral blood leucemic cells and bone marrow aspirate, peripheral blood lymphocytes and the number of stem CD34+ cells

  • Clinical autopsy and the coroner’s office

Department for pathology

Chief of the department: Ass. Prof. Zinaida Karasalihović, specialist in anatomical pathology

Phone: ++ 387 35 303 535

e-mail: zinaida.karasalihovic@ukctuzla.ba

Nurse in charge: Sanela Imamović, engineer of medical laboratory diagnostics

Phone: ++ 387 35 303 570

e-mail: sanela.imamovic@ukctuzla.ba

Health care services:

  • processing and standard microscopic diagnostic on small tissue samples obtained by endoscopic methods (esophago-gastroscopy, enteroscopy, colonoscopy, cystoscopy, laparoscopy, hysteroscopy, bronchoscopy, etc.),
  • processing and standard microscopic diagnosis of samples obtained by needle biopsy (kidney, liver, tumors of various systems, breast tumors) and experimental surgical biopsies,
  • processing and standard microscopic diagnostics on material obtained after surgical interventions,
  • processing and microscopic diagnostics on cryostat sections of fresh tissue for urgent intraoperative analysis of surgical samples, for microscopic analysis of small samples of skin, muscles, nerves and needle samples of the liver and kidney for transplantation rejection diagnosis,
  • application of special histostaining (PAS, Gomori trichrome, Mallory trichrome, van Gieson-Weigert, Masson’s trichrome, Congo red, Sudan black, Oil red, Prussian blue, reticulin Jones, etc. )
  • application of immunohistochemical tissue stains using the EnVision method and thre- step immunoperoxidas with spreptavidin, with more than 200 specific, primary antibodies,
  • application of direct immunofluorescence method for diagnosis of immune deposits of immunoglobulins and complement components on fresh tissue samples of skin, muscles, nerves and needle samples of the liver and kidney for transplantation rejection diagnosis,
  • clinical autopsies and the coroner’s office

Department for citology

Chief of the department: Dr. Maida Kuljanin, MSc., specialist in anatomical pathology

Telephone: ++ 387 35 303 566

E-mail: maida.kuljanin@ukctuzla.ba

Nurse in charge: Selma Hasanović, MSc., graduated nurse

Telephone: ++ 387 35 303 537

E-mail: selma.hasanovic@ukctuzla.ba

Health care services

  • Processing and analysis of fine- needle aspiration biopsy (FNA) of the thyroid gland and tumors of various organs, including fine- needle aspiration biopsy of cystic breast lesions, as well as tranchbronchial samples obtained during bronchoscopy.

  • Processing and analysis of exfoliative samples of all organs and and body fluids, including a PAP smears (exfoliative cytology).

  • Processing of punctures obtained from body cavities and production of a paraffin block from the obtained material.

  • Special histochemical and immunohistochemical stains on cytological samples, which enables the analysis of cellular markers and the diagnosis of undifferentiated malignant tumors and the subclassification of individual tumors.

Department for molecular pathology

Chief of the department: Prof. Dr, Elmir Čičkušić

Telephone: ++ 387 35 303 508; 303 509
e-mail:elmir.cickusic@ukctuzla.ba

Nurse in charge: Jasmina Ovčina MSc., graduated nurse

Telephone: ++ 387 35 303 549 i 303 509
e-mail: jasmina.ovcina@ukctuzla.ba

Services of laboratory for molecular diagnostics

  • DNA extraction from peripheral blood cells, buccal mucosa, tissue samples taken by biopsy or from formalin- fixed and paraffin- embedded tissues.
  • DNA HLA antigen typing class I and class II at a lower level of genetic resolution using the PCR- SSOP method.
  • DNA typing at an intermediate level of genetic resolution using the PCR- SSP method.
  • DNA typing of HLA antigens at a high level of resolution using the method of sequence analysis (PCR- SBT)
  • Chimerism detection by analysis of 15 STR gen loci and amelogenin in patients after hematopoietic stem cell transplantation
  • Identification of 19 mutations of CFTR gene for molecular diagnosis of cystic fibrosis.
  • Paternity testing by identifying 16 STR gene loci
  • Detection and typing human papillomavirus (HPV) in cervical swabs by molecular methods that detect HPV DNA,the Digene HPV DNA Test
  • Chromogenic in situ hybridisation for the assessment of HER2 status in breast cancer
  • Qualitative RT- PCR analysis for the detection of the PML- RAR and BCR- ABL fusions
  • Quantitative RT- PCR measurement of the trancript levels BCR- ABL gene
  • Qualitative analysis of genes responsible for resistance to imatinib mesylate (Gleveek)
  • Trombophilia Genetic Panel

Laboratory for cytogentics

  • Karyotype analysis from peripheral blood lymphocytes using G banding technique.
  • Karyotype analysis from bon marrow cells
  • Karyotype analysis from amniotic fluid cells
  • Karyotype analysis from chorionic villi
  • Karyotype analysis from cord blood

Laboratory for flow cytometry

  • Flow cytometric immunophenotyping of leukemia cells in peripheral blood and bone marrow aspirate
  • Flow cytometric immunophenotyping of peripheral blood lymphocytes
  • flow cytometric enumeration of CD34+hematopoietic stem cells

INSTITUTE FOR MICROBIOLOGY

Chief of institute:

Telephone: ++ 387 35 303 536

E- mail:

Nurse in charge: Mubera Kutlovac, higher laboratory technician

Telephone: ++ 387 35 303 568

E-mail: mubera.kutlovac@ukctuzla.ba

Reception

Telephone: ++ 387 35 303 511

Information on findings:

Telephone: .++ 387 35 303 511; working hours: 12 noon- 2 pm

The Institute of Microbiology has three main areas of activity: diagnostics, education and scientific research. Every year, the Institute for Microbiology tests 88,822 patients, that is, 109,923 different samples, or 285, 544 different analyses. At the institute health professionals have examined the various materials for the diagnosis and treatment of diseases caused by bacteria, fungi, parasites and viruses, as well as the diagnosis of autoimmune and allergic diseases and the assessment of the immune status of patients. Our expertise is the monitoring of bacterial resistance and antimicrobial agents, and the monitoring of hospital infections. For diagnostic purposes, we have used various methods from the fields of bacteriology, virology, parasitology, mycology, immunology, molecular medicine, hospital hygiene and sanitary microbiology such as:

  • Direct microscopic examination of patient material
  • Cultivation of aerobic and anaerobic bacteria, cultivation of mycobacteria and fungi on nutrient media for identifying pathogenic microorganisms
  • Examination of the sensitivity of isolated bacteria and fungi to antimicrobial agents
  • Immunological diagnostics for the detection of antibodies and antigens
  • Molecular diagnostics

Microbial identification is done by:

  • The innovative VITEK 2 Compact microbial identification system
  • Fast microbial identification which enables timely diagnosis
  • Sensitivity to antimicrobial agents enables clinicians to choose the most adequate antibiotic treatment

Determining the antibiogram on the VITEC2 Compact:

  • Reading of the results is done automatically by the Expert system for the validation of the sensitivity test
  • A possibility of identifying potential mechanisms of resistance, even emerging of low- level resistance. It contributes to the detection of nosocomial infections.

Department for mycrobacteria, is one of the biggest laboratory in our country

  • Direct microscopy
  • Culturing Mycobacterium tuberculosis obtained from patient’s material on solid media
  • Culturing Mycobacterium tuberculosis on liquid media using BACTEC MGIT 960
  • Drug susceptibility testing to first- line antituberculosis drugs
  • Typing of mycrobacteria

The following tests are used for immunological diagnostics:

  • Enzyme linked immunosorbent assays (ELISA)
  • Immunofluorescence tests
  • Western Blot Test
  • Nephelometric tests
  • Agglutination tests

Detection of antibodies and antigens to bacteria, viruses, fungi and parasites

  • It is performed on a BEP 2000 ELISA system that provides the detection of specific antibodies against microorganisms in human serum or plasma.
  • Results are expressed in IU/ml or as antibody titers
  • Antibodies of the IgM, IgG and IgA class can be determined, which show functional differences of an acute infection from chronic infection

Detection of antibodies on the BEP 2000 ELISAsystem is performed for:

  • Brucellae, Coxiella burnetti, leptospirae, Helicobacter pylori, Chlamydie, Mycoplasma pneumoniae
  • CMV, HSV type 1 and 2, VZV, EBV, Rubella virus, HIV, HBV, HCV and HAV markers, Parvovirus B 19, Morbilli virus, Mumps virus, Adeno virus, Coxsackiae
  • Toxoplasma gondii, Trichinella spiralis, Cysticercosis, Echinococcus

The automated ELISA processor HYTEC 288 PLUS is used for the diagnosis of autoimmune and allergic disease:

  • The detection and quantification of antibodies in serum: Systemic Lupus Erythematodes (SLE), Sjögren’s Syndrome (SS), Poly/Dermatomyositis, Scleroderma, CREST.
  • The detection and quantification of organospecific antibodies
  • The detection of allergen – specific IgE antibodies ( inhalation allergens, food allergens and drugs)

In autoimmune and systemic diseases, the Luminex Xmap technology is used to determine several parameters in one serum sample

  • Antinuclear Antibodies (ANA)profile 9- biomarkers

Detection of HLA reactive antibodies in organ transplant recipients at Luminex.

Immunofluorescence tests are also used in routine diagnostics for:

  • Detection of Chlamydia trachomatis in urethral and Pap smears
  • Detection of antibodies to Listeria monocytogenes
  • Detection of antibodies to Bartonella henselae (cat- scratch disease)
  • Detection of antibodies to Chlamydia trachomatis, Chlamydia pneumoniae and Chlamydia psittaci
  • Detection of antibodies in serum against the causative agents of myocarditis (Myocarditis profile)
  • Detection of antibodies in serum against the causative agents of respiratory infections
  • Detection of antibodies in serum and cerebrospinal fluid to the causative agents of central nervous system infections (CNS profile)
  • Anti- nuclear antibodies
  • Anti ds DNA antibodies
  • Anti SMA antibodies
  • Anti- sperm antibodies

The Western blot test is an antibody test is used to confirm or disapprove the results:

  • Confirmatory test for IgM and IgG antibodies reactivity to antigens of Treponema pallidum
  • Confirmatory test for IgM and IgG antibodies reactivity to antigens of Borellia burgdorferi
  • Confirmatory test for IgM and IgG antibodies reactivity to antigens of Borellia afzelii
  • Confirmatory test for IgM and IgG antibodies reactivity to antigens of Borellia garinii
  • Confirmatory test for IgM and IgG antibodies reactivity to antigens of Hantavirus
  • Profile for diagnosis of autoimmune hepatitis
  • ANA profile

Agglutination tests for rapid diagnosis:

  • The Rose Bengal for diagnosis of Brucellosis
  • TPHA for diagnosis of syphilis
  • IHA for Leishmaniasis
  • IHA for amebiasis
  • Anticardiolipin antibody for syphilis

Molecular diagnostics enables faster and more accurate results in comparison to traditional microbiological methods. At present, molecular techniques include quantitative PCR (real-time PCR), DNA microarray and in situ hybridization. The COBAS TaqMan System and Light Cycler and GenExpert system are molecular platforms that automates detection of DNA or RNA for different organisms:

  • Detection of HCV RNA and HIV RNA, HBV DNA and CMV DNA, EBV DNA, Parvovirus DNA, BK virus Testing for Clostridium difficle in the stool and detection of Enterovirus in the cerebrospinal fluid using PCR method.
  • HCV genotyping
  • HBV genotyping
  • HBV drug resistance

The institute is a teaching and academic base for the education of health- oriented profiles such as high medical school students, senior laboratory technicians, students of medical faculty. Pharmaceutical and technology students, doctors specializing in microbiology, infectology, dermatovenerology, epidemiology and clinical immunology. Continuous education and professional development are extremely important and the essential components of our institute strategy.

As a result of a scientific research work of our doctors, laboratory technicians and biologists 7 doctoral dissertations and 13 master’s theses have been completed, 6 books have been written and 250 papers published. Our academic workforce is consisted of one full professor, one associate professor, two assistant professors and five assistants.

Organizing structure

Department for general microbiology

Department for microbacteriology and respiratory infections

Department for clinical immunology

Department for microbiology, virology with serology

Department for general microbiology

Chief of the department: Prof. Dr. Fatima Numanović, specialist in microbiology with parasitology

Telephone: ++ 387 35 303 564

E-mail:fatima.numanovic@ukctuzla.ba

Laboratory technician in charge: Jasmina Bećirović, engineer of medical laboratory diagnostics

Telephone: ++ 387 35 303 517

E-mail: jasmina.becirovic@ukctuzla.ba

1. Laboratory for bacteriology:

  • The following samples are analyzed: eye and ear swabs, urethral swabs, semen, vaginal and cervical swabs, vulva swab, wound swab, prepuce swab, skin changes, catheters and drains, and other material.

  • Isolation and identification of aerobic and anaerobic bacteria are performed using standard microbiological methods(microscopically, culture, biochemical and serological)

  • Identification of aerobic, anaerobic and slow- fermenting bacteria using the Vitek 2 system

  • Monitoring the resistance of microorganisms (intrahospital strains) to antibacterial agents

  • Microbiological examinations: urine and stool for pathogenic aerobic and anaerobic bacteria and their sensitivity to antibiotics

  • Detection of Adenovirus and Rotavirus in the stool sample

  • Detection of Helicobacter pylorii antigen in the stool sample

  • The fecal occult blood test

  • Assessment of bacterial flora of the gut

  • Isolation and identification of aerobic and anaerobic bacteria in the stool sample using standard microbiological methods

  • Macroscopic and microscopic detection of intestinal parasites in the stool sample

  • Standard methods in parasitology: native and colored preparation, cellophane print of the perianal region, stool concentration methods; sedimentation- flotation methods MIF, and methods of concentration of Strongiloides stercoralis larvae.

Protozoa of body cavities are diagnosed:

  • Examination of the native preparation, preparation stained with acridine orange, preparation prepared by the MIFC method and examination of the direct stool preparation in eosin solution.
  • Blood-tissue protozoa are detected by microscopic examination of blood smears, biopsy material and thick drops, or directly by immunofluorescence.
  • Isolation of yeasts, molds and dermatophytes is done by cultivating different material (throat and nose swabs, sputum, skin changes, scales from changed skin and nails, hair, sperm, urethral swabs, vaginal and cervical swabs), and detection is performed using standard mycological methods. (macroscopic, microscopic and biochemical), their examination. sensitivity to antifungal drugs.
  1. Department for microbacteriology and respiratory infections

    Chief of the department: Prim. Dr. Hanka Kikanović, specialist in microbiology with parasitology

    Telephone: ++ 387 35 303 5717

    E-mail:hanka.kikanovic@ukctuzla.ba

    Laboratory technician in charge: Semina Gazibgović, engineer of medical laboratory diagnostics

    Telephone: ++ 387 35 303 517

    E-mail:semina.gazibegovic@ukctuzla.ba

Laboratory for microbacteriology and respiratory infections:

  • Analysis of the following samples: throat and nose swabs, examination of sputum, aspirate, and punctuate, blood, cerebrospinal fluid, valves.
  • Detection and identification of aerobic and anaerobic bacteria is performed by standard microbiological methods (microscopic, cultural, biochemical and serological).
  • Identification of aerobic, anaerobic, slow-fermenting bacteria is performed on the Vitek 2 and Bactec system for blood cultures
  • Monitoring the resistance of microorganisms (intrahospital strains) to antibacterial agents
  • Detection of mycobacterial tuberculosis and other mycobactria in biological materials
  • Examination of the direct preparation according to Ziehl- Nelsen staining technique, material homogenization, examination of the preparation after staining and homogenization
  • Cultivation of homogenized and neutralized material on Loewenstein substrates.
  • Cultivation of biological materials on the Bactec system, growth identification and identification of Mycobacterium tuberculosis.
  • Resistance testing by the method of Cannetti et al. to Isoniazid, Streptomycin, Rifadin, Ethambutol, Pyrazinamide and other antituberculosis drugs.
  • Typing of grown cultures of atypical mycobacteria and examination of their sensitivity to other antimicrobial agents.
  • Introduction of genetic tests in the diagnosis of mycrobacteria
  • Examination of resistance and differentiation of mycobacteria using the Bactec method.
  • Performing a polymerase chain reaction to prove mycobacteria in cerebrospinal fluid, urine and various punctuates.
  • Determination of Mycobacterium tuberculosis resistance.

  • Keeping records

  • Release of results

  • Investigation of various possibilities for better isolation, detection and identification of mycobacteria.

  • Monitoring and analyzing findings, results and reporting.

  1. Department for clinical immunology

    Chief of the department: Prof. Dr. Fejzo Džafić, specialist in clinical immunology

    Telephone: ++ 387 35 303 563

    E-mail:fejzo.dzafic@ukctuzla.ba

    Technician in charge: Kadrija Hadžimehmedović, engineer of medical laboratory diagnostics

    Telephone: ++ 387 35 303 563

    E-mail:kadrija.hadzimehmedovic@ukctuzla.ba

Laboratory services:

  • Serologic testing for autoantibodies in the serum of patient for the diagnosis, differential diagnosis, prognostication, or monitoring of autoimmune diseases.

  • Determination of immune status

  • Diagnosis of allergic diseases

  • Panel reactive antibodies (PRA)

Methods and techniques:

  • Immunofluorescence test

  • Immunoenzymatic test- LISA

  • nephlometric method

  • Luminex-x MAP technology

  • Immunoblot method

Tests:

  • The indirect IF test detects and determines the titer of the following autoantibodies: ANA, Anti dsDNA, ASMA and antisperm antibodies.

  • The ELISA test detects and determines the titer of antibodies to: AMA, LKM, dsDNA, ANA, cardiolipin, antigliadin antibodies IgG, antiendomysial antibodies IgA, GBM, pANCA, cANCA, anti CCP, interleukins, GAD antibodies, insulin antibodies, CIC, gastric parietal antibodies.

  • The nephelometric method measures the concentration of immunoglobulins – IgG, IgM, IgA, IgE classes; complement system components C3 and C4, RF.

  • The immunoenzymatic test determines specific IgE to nutritional, inhalation and occupational allergens, drugs and insect poisons.

  • ANA profile and liver profile are done by immunoblot method.

  • The Luminex method is used for detection and quantification in the following autoimmune panels: ANA (9 parameters ANA profile) Panel Reactive Antibodies (PRA )

4. Department for molecular microbiology, virology with serology

Chief of the department: Primarius Dr. Zineta Delibegović, specialist in microbiology with parasitology

Telephone: ++ 387 35 303 561

E-mail:zineta.delibegovic@ukctuzla.ba

Technician in charge: Mirel Suljetović, engineer of medical laboratory diagnostics

Telephone: ++ 387 35 303 563

E-mail: mirel.suljetovic@ukctuzla.ba

Laboratory for molecular microbiology

Laboratory for virusology

Laboratory for serology

Laboratory services:

  • Indirect proof of infections with bacteria, fungi, parasites and viruses by methods of proving antibodies in serum
  • Direct proof of infections by detection of nucleic acids of microorganisms using the method of hybridization and amplification of nucleic acids (PCR)

Methods and techniques:

  • Immunofluorscence test
  • ELISA test
  • Immunochromatographic tests
  • Western blot/ Immunoblot tests
  • Agglutination reactions
  • Precipitation reactions
  • Nephelometric method
  • Chemiluminescence tests
  • PCR
  • PCR/hybridization tests
  • Real- time PCR

Tests:

  • The agglutation reaction detects antibodies to brucellosis
  • The precipitation reaction determines anticardiolipin antibodies (RPR)
  • Nephelometry to determine AST
  • The indirect IF test detects antibodies to: respiratory pathogens, pathogens that cause CNS infections, myocarditis, respiratory infections and determination of antibody titers in serum for Chlamydiae, Listeriae, Legionellae, Bartonella haenselae, Ricketsiae
  • The ELISA test detects and determines the titer of antibodies to: CMV, EBV, Rubella virus, Morbilli virus, HSV 1 and 2, Mumps, Parvo virus B19, Enteroviruses, Adenoviruses, Coxsackie viruses, Hantan virus, West Nile virus, viral hepatitis markers HAV , HBV, HCV, Brucellae, Coxiella, Francisella, Helicobacter, Leptospirae, Borreliae, Bordetella, Mycoplasma, Echinococcus, Cysticercosis, Toxoplasma, Toxocara, Trichinella, Candida, Apergillu
  • Westernblot as a conirmatory test for infection with Borrelia burgdorferii sensu lato, Treponema pallidum.

  • Immunoblot tests for Hantan viruses,

  • Real time PCR detects and quantifies nucleic acids for viruses and bacteria: CMV, HBV, HCV, HIV, EBV, Parvovirus, JC/BK virus, Enteroviruses, Clostridium difficile, Chlamydia trachomatis.

  • Genotyping of HCV, HBV

  • Resistance to Lamivudine and Entecavir for HBV

  • HBV Precore/Core mutations

  • QuantiFERON-TB Gold test (QFT)

INSTITUTE FOR BIOCHEMISTRY

Chief of Institute for biochemistry: Ass. Prof. Fatima Hukić, specialist in medical biochemistry

Telephone: ++ 387 35 303 502

E-mail: fatima.hukic@ukctuzla.ba

Technician in charge: Nisveta Puran, higher laboratory technician

Telephone: ++ 387 35 303 525

E-mail: nisveta.puran@ukctuzla.ba

Institute for Biochemistry offers specialty biochemistry and hematological testing and laboratory diagnostics. Working hours is 24/7. Our laboratory services provide about 200 different tests processed using state-of-the-art technology selected according to IFCC (International Federation of Clinical Chemistry and Laboratory Medicine) standards, and highly trained professional staff for the benefit of the entire health community.

Since 2007, as part of a leading academic hospital, the Institute has pursued teaching and research activities and provided provide teaching in laboratory medicine for our students and residents and other healthcare professionals.

ORGANIZING STRUCTURE

Department for Biochemistry

Department for Hematology

Department for urgent program

Department for biochemistry

Chief of the department: Alma Osmić Husni, MSc., specialist in medical biochemistry

e-mail: alma.osmichusni@ukctuzla.ba

Laboratory technician in charge: Dženuma Suljkanović, higher laboratory technician

e-mail: dzenuma.suljkanovic@ukctuzla.ba

Laboratory:

Telephone: ++ 387 35 303 516; 303 572

The department has several teams analyzing substrates, enzymes, proteins, electrolites and ABS, toxicology, lipids and vitamins, hormones and urine.

Laboratory services:

  • Laboratory diagnostics of biochemical parameters: glucose in serum and cerebrospinal fluid, urea, creatinine, urate, total, conjugated and unconjugated bilirubin, neonatal bilirubin using the kinetic spectrophotometry method, recommended by the IFCC;
  • Determination of HbA1c and parameters of blood glucose regulation by immunochemical technique, according to IFCC;
  • Determination of enzymes: ALP, LDH, amylase, pseudocholinesterase, GGT, lipase, by kinetic spectrophotometry in the UV region, according to IFCC;
  • Laboratory diagnosis of myocardial infarction: troponin, myoglobin, AST, ALT, CK, CKMB, by immunochemical method, kinetic spectrophotometry method, immunoturbidimetry in the UV region, according to IFCC;
  • Determination of minerals: Ca, P, Na, K, Mg, Cl, Fe, TIBC, UIBC, Cu by the method of kinetic spectrophotometry and indirect potentiometry, according to IFCC;
  • Determination of the concentration of antiepileptic drugs in the blood: phenobarbitone, carbamazepine, valproic acid, phenytoin using the modern CMIA method
  • Determination of immunosuppressants: ciclosporin and tacrolimus using the latest CMIA technique (improved chemiluminescence method);
  • Determination of tumor markers: CEA, AFP, TPSA, free PSA, Ca15-3, Ca19-9, parathyroid hormone, vitamins, B12 and D vitamins (CMIA method);
  • Assessment of the risk of artherosclerosis by measuring cholesterol, triglycerides, HDL and LDL cholsterol, APO A and APO B lipoproteins using kinetic spectrophotometry and immunophelometry;
  • Determination of Igg, crebrospinal fluid, κ and λ light chains in serum and urin, fibrinogen, microalbumin, ferritin, transferrin, α1-antitrypsin, haptoglobin by immunonephelometry method;

  • Dtermination of metabolites hom-5-HIAA, porphobilinogen, -δ-aminolevulinic acid, cysteine, ß 2-microglobulin (CMIA, turbidimetry)

  • Examination of urine, spermogram, occult blood test in fecal, the fecal fat test

  • Determination of hydroxy and ketosteroids, total porphyrins, coproporphyrins, uroporphyrins, in 24-hour urine in microchromatographic columns;

  • Chemical analysis of stone and metabolic screening of urine.

Dpartment for Hematology

Chief of the department: Primarius MPh. Selma Imširagić Zovko MSc, specialist in medical biochemistry

Telephone: ++ 387 35 303 573

e-mail: selma.zovko@ukctuzla.ba

Technician in charge: Irfan Hadžić, engineer of medical laboratory diagnostics

Telephone: ++ 387 35 303 573

E-mail: irfan.hadzic@ukctuzla.ba

The department has two teams for automatic analyzers and special coloring. The department offers laboratory diagnostics of the blood system and monitoring of disorders of hematological disases.

  • Anemia
  • Benign isases of granulocytes and monocytes
  • Malignant tumors of granulocytes and monocytes
  • Acute leukmia
  • Benign diseases of lymphocytes
  • Neoplastic proliferation of lymhocytes
  • Platelet diseases
  • Hematopietic stem ceell transplantation (smear staining and mononuclar count)
  • Cytochemical tests
  • Complete examination of the cerebrospinal fluid
  • Sedimentation

The laboratory is equipped with a new generation of automatic counters: Sysmex XE-N1000-DIF), Sysmex XP300 (3DIF), Beckman COULTER DxH, ABBOTT-RUBY (5DIF). The parameters measured are: WBC, RBC, HGB, HCT, MCV, MC, MCHC, PLT, RDW-SD, RDW-CV, PDW, MPV, P-LCR, PCT, NEUT, LYMPH, MONO, EO , BASO, NRBC, RET, IRF, LFR, MFR, HFR, RET-He.

Basic techniques of staining peripheral smears and their differentiation by microscopic method are used. Smears are made and stained according to already adopted procedures (May-Grunwald-Giemsa, Sudan, etc.)

Hematology quality control procedures for checking the technical precision and accuracy of the hematology counter and the results are routinely performed in daily work. External quality control is performed for differential leukocyte formula- DKS.

Departmnt for urgent program

Chief of the department: Primarius MPh Nada Skokić, specialist in medical biochemistry

Telephone: .++ 387 35 303 516

e-mail: nada.skokic@ukctuzla.ba

Laboratory services:

Radiometer-ABL 800, ABL 5 and Cobas b 221 are multiparameter analyzers that determine: parameters of gas analysis, homeostasis of hydrogen ions (pH), ionized electrolytes, oximetry and metabolic parameters. Depending on which parameter is measured on the mentioned analyzers, they are used different measuring principles (potentiometric, amperometric or optical).

Group of parameters:

pH/gas u krvi PH, PCO2, pO2

Oximetry: ctHb (total hemoglobin concentration); sO2 (oxygen saturation); FO2Hb (fraction of oxyhemoglobin in total hemoglobin); FCOHb (fraction of carboxyhemoglobin in total hemoglobin); FHHb (fraction of deoxyhemoglobin in total hemoglobin); FmetHb (fraction of methemoglobin in total hemoglobin); FHbF (fraction of deoxyhemoglobin in total hemoglobin.

Electrolytes: cK+ (potassium ion concentration); cNa+ (sodium ion concentration); cCa2+ (calcium ion concentration); cCl-( chloride ion concentration)

Substrates: cGlu (D-glucose concentration); cLac (lactate concentration); ctBil (total bilirubin concentration in plasma); cCrea (blood creatinine concentration)

On the biochemical analyzer Dimension RXL Max, ARCHITECT-i8200, BC-AU480, which works on the principle of spectrophotometric measurement, following analyzes are performed:

Substrates in serum and urine

Glucose in serum and CSF: GLUC (glucose concentration in serum or CSF)

Urea in serum and urine: BUN (Blood Urea Nitrogen) concentration of urea in serum and urine

Serum bilirubins: TBIL concentration of total bilirubin in serum; DBIL serum direct bilirubin concentration

Creatinine in serum and urine: CREA creatinine concentration in serum and urine

Ammonia: Concentration of ammonia in plasma

Lactate: Concentration of lactate in plasma

Enzymes in serum: AMY (amylase activity in serum and urine); ALT (alanine aminotransferase activity in serum); AST (aspartate aminotransferase activity in serum)

Cardiac parametrs: LDH (Lactate dehydrogenase activity in serum), CK (creatine kinase activity in serum), MMB – Mass Creatine Kinase MB- principle of EIA-enzyme immunoassay CTNI- troponin I-principle EIA-enzyme immunoassay

Proteins in serum and cerebrospinal fluid: TP – total proteins – Biuret modification ALB- albumins- Bromcresol Purple RCRP – turbidimetric immunoassay (PETIA)

Electrolites: cK+ (potassium ion concentration)-IMT-indirect method, cNa+ (sodium ion concentration)-IMT-indirect method, cCa2+ (concentration of calcium ions) – o-Cresolphthalein complex, cCl-( chloride ion concentration)- IMT-indirect method

Architect i2000, by immunochemistry chemiluminescence method (CMIA)

Hormones and tumor markers, immunosuppressant: β-HCG, human chorionic gonadotropin; Tacrolimus

AXSYM (fluorescence polarization principle, FPIA) Spectrophotometrically

Concentration of drugs in serum and immunosuppressive drugs Carbamazepine Phenobarbitone Valproic acid Cyclosporine (whole blood) Salicylates in serum

Viva E (EIA method)

Addictive substances in urine Alcohol (urine and blood): Barbiturates Methadone Opiates Benzodiazepine Amphetamine Cannabinoids Tricyclic antidepressants Cocaine and cocaine metabolites Phenylcyclidine Ecstasy

On the Sysmex XP300 hematology analyzer using three detector blocks, 19 hematological parameters are determined: WBC, LYM, MXD, NEUT, RC, HGB, HCT, MCV, MCH, MCHC, RDW-CV, RDW-SD, PLT , PDW and MPV.

Physical and chemical examination of urine (test strips) and microscopic examination of urine sediment. Modern analytical equipment and continuous internal and external quality control ensure reliable laboratory findings during 24 hours.